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KMID : 0895820030130040147
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Journal of Oriental Rehabilitation Medicine
2003 Volume.13 No. 4 p.147 ~ p.171
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The effect of Cirsii Japonici Herba on gene expression profile of HepG2 cells oxidative-stressed with hydrogen peroxide
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Cha Yun-Yeop
Kim Sung-Su
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Abstract
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Objectives: In recent years, We are concerned in disease protection, anti-aging, longlife, and many method are used in solving this problem, for example, Natural Detoxificaton therapy in point of Alternative medicine. Among that, ’Milk thistle’ protect a pollutant, chemistry material, radiation, poison of alcohol about Liver and used in ant-oxidant’s matter. It is proved that Cirsii Japonici Herba(CJH) grew up in Korea is better than other regions in point of detoxification-liver function, ant-oxidant’s working.
Methods: The purpose of this study is to; 1) evaluate the recovery of damage by assessing the anti-oxidant effect of Cirsii Japonici Herba(CJH) on HepG2 cells, 2) identify candidate genes responsible for anti-oxidative effect on HepG2 cells by oligonucleotide chip microarray. The viability of cells treated with CJH was not significantly recovered from stressed HepG2 cell. But, wide range of concentrations of CJH shows dose-dependent inhibitory effect on ROS production in oxidative-stressed cells. Total RNAs of cells without treatment (Control group), only treated with H2O2 (Stressed group) and treated with both H2O2 and of CJH (CJH group) were isolated, cDNAs was synthesized using oligoT7-(dT)24 primer. The fragmented cRNAs, synthesized from cDNAs, were applied to Affymetrix GeneChip. Twenty-three kinds of genes were downregulated, whereas nine kinds of genes were upregulated in Stressed group comparing to Control group. Thirteen kinds of genes were downregulated, whereas ten kinds of genes were upregulated in CJH group comparing to Control group. Six kinds of genes were downregulated, whereas Twenty-five kinds of genes were upregulated in CJH group comparing to Stressed group.
Result and Conclusions: The results suggest that decreased viability and ROS production of HepG2 cell by H2O2 may be, at lease, mediated by interfere with EGF signaling on oxidative stress of Epidermal growth factor receptor pathway substrate 15 (EPS15), and Four kinds of ITPR2, CYR61, NCKAP1, NR3C1 play an important role of aging control, because of the improvement of cell metabolic function. The list of differentially expressed genes may implicate further insight on the action and mechanism behind the anti-oxidative effects of herbal extract Cirsii Japonici Herba.
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KEYWORD
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Cirsii Japonici Herba, anti-oxidative effect, HepG2 cell, Oligonucleotide Chip Microarray, anti-aging
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